OKAU00114 | Glucose Colorimetric Detection Kit Biotrend

Datasheets/Manuals	Printable datasheet for Glucose Colorimetric Detection Kit (OKAU00114)

Product Info

ELISA Kit Detection Method

Colorimetric

ELISA Kit Linearity

Linearity was determined in human serum and plasma samples by taking two diluted samples with known glucose concentrations. One serum sample had a high glucose concentration of 10.7 mg/dL and one had a lower value of 3.67 mg/dL. One plasma sample had a high glucose concentration of 5.42 mg/dL and one had a lower value of 2.25 mg/dL. They were each mixed in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used. Serum Linearity

Low Sample	High Sample	Expected Conc. (mg/dL)	Observed Conc. (mg/dL)	% Recovery
80%	20%	5.09	4.61	90.6
60%	40%	6.51	6.49	99.8
40%	60%	7.93	7.79	98.3
20%	80%	9.35	9.26	99.0
			Mean Recovery	96.9%

Plasma Linearity

Low Sample	High Sample	Expected Conc. (mg/dL)	Observed Conc. (mg/dL)	% Recovery
80%	20%	2.89	2.69	93.2
60%	40%	3.52	3.68	104.4
40%	60%	4.15	4.05	97.5
20%	80%	4.79	5.10	106.6
			Mean Recovery	100.4%

ELISA Kit Principle

The DetectX® Glucose Colorimetric Detection Kit is designed to quantitatively measure glucose in a variety of samples. Please read the complete kit insert before performing this assay. A b-D-glucose standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the Colorimetric Substrate and horseradish peroxidase and the reaction initiated by addition of glucose oxidase. The reaction is incubated at room temperature for 30 minutes. The glucose oxidase reacts with glucose to produce hydrogen peroxide which, in the presence of HRP, reacts with the Substrate to convert the colorless substrate into a pink-colored product. The pink product is read at 560 nm. Increasing levels of glucose cause a linear increase in color.

ELISA Kit Reproducibility

Intra Assay Precision Three diluted human serum samples were run in replicates of 20 in an assay. The mean and precision of the calculated concentrations were:

Sample	Glucose Conc. (mg/dL)	%CV
1	13.96	4.1
2	9.54	3.4
3	1.78	10.5

Inter Assay Precision Three diluted human serum samples were run in duplicate in seventeen assays run over multiple days by three operators. The mean and precision of the calculated concentrations were:

Sample	Glucose Conc. (mg/dL)	%CV
1	13.19	11.2
2	9.40	6.4
3	1.59	9.4

ELISA Kit Component

Component	Quantity
Clear Half Area 96 Well Plates	2 Plates
Glucose Standard	90 uL
Assay Buffer	50 mL
Substrate	5 mL
Horseradish Peroxidase Concentrate	60 uL
Glucose Oxidase Concentrate	600 uL

Additional Information

Background: Glucose (C6H12O6) is by far the most common carbohydrate. It is a monosaccharide, an aldose, a hexose, and a reducing sugar and is also known as dextrose, because it is dextrorotatory (rotates polarized light clockwise). The structure of glucose is shown below as both the straight chain and cyclic forms. For all biological and molecular events and for multiple cellular functions, energy is essential. Energy is available in the form of ATP (adenosine triphosphate), most of which is generated through aerobic cellular respiration of carbohydrate and glucose, the major source of biological free energy in higher organisms. Reduced energy levels threaten cellular homeostasis and integrity. Impaired energy metabolism may trigger pro-apoptotic signaling (programmed cell death), oxidative damage, excitotoxicity and impede mitochondrial DNA repair. A serious fall in blood glucose can be characterized by metabolic dysfunction, neuroglycopenia, seizure, and death. A persistent elevation in blood glucose leads to "glucose toxicity." Glucose toxicity contributes to ß-cell dysfunction and the pathology grouped together as complications of diabetes. Estrogen-induced signaling pathways in hippocampal and cortical neurons involve the mitochondria to enhance mitochondrial function and to sustain aerobic glycolysis and citric acid cycle oxidative phosphorylation and ATP generation.

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Detection Limit: 0.304 mg/dL

Reconstitution and Storage

2°C to 8°C

Sample Type

Serum, Plasma, Urine, Buffers and TCM

Sensitivity

0.413 mg/dL

Gene Full Name	Glucose Colorimetric

Glucose Colorimetric Detection Kit

Katalog-Nummer OKAU00114

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Marke : Aviva Systems Biology

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