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Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Gibberellic acid [77-06-5]

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Katalog-Nummer HY-N1964-1ml

Size : 10mM/1mL

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Telefonnummer : +1 850 650 7790

Marke : MedChemExpress

Contact local distributor :

Telefonnummer : +1 850 650 7790

Gibberellic Acid is named after the fungus Gibberella fujikuroi. Gibberellic Acid regulates processes in plant development and growth, including seed development and germination, stem and root growth, cell division, and flowering time. Gibberellic Acid also improves plant response to growth stress caused by various environmental stresses, such as cold stress, drought stress, heavy metal stress, etc. Gibberellic Acid also causes increased lipid peroxidation and fluctuations in the antioxidant defense system in rats.

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Gibberellic acid Chemische Struktur

CAS. Nr. : 77-06-5

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Other Forms of Gibberellic acid:

Gibberellic acid (Standard) Angebot einholen

Beschreibung

In Vitro

Gibberellic acid rescues chickpea seedlings from cold stress. Gibberellic acid (10-20 μM) increases final emergence probability (FEP), helping seeds maintain higher relative water content and lower electrolyte leakage^[2].
Gibberellic acid (0.025 μM) treatment of Echinacea purpurea L. hairy roots increased PAL activity, cell viability, and free radical scavenging activity in hairy root cultures. Gibberellic acid optimizes the production of secondary metabolites in Echinacea purpurea L. hairy root cultures and is beneficial to the growth of hairy roots^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Rats were exposed to 75 ppm ABA and GA3 ad libitum for 25 days as drinking water, the PGRs intake amount of each rat was about 2.2 mg/day^[5].
Gibberellic acid (2.2 mg/rats/day; po) can promote the content of lipid peroxidin and change the antioxidant system in various tissues of rats^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molekulargewicht

346.37

Formel

C₁₉H₂₂O₆

CAS. Nr.

77-06-5

Appearance

Solid

Color

White to off-white

SMILES

OC([C@H]1[C@@]([C@]23C)(10)[C@@](OC2=O)(C=C[C@@H]3O)[C@@](CC4)(10)[C@]1(C5)C[C@]4(O)C5=C)=O

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (288.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8871 mL	14.4354 mL	28.8709 mL
5 mM	0.5774 mL	2.8871 mL	5.7742 mL
10 mM	0.2887 mL	1.4435 mL	2.8871 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner
Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Reinheit & Dokumentation

Purity: ≥98.0%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (253 KB) HNMR (255 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8871 mL	14.4354 mL	28.8709 mL	72.1772 mL
	5 mM	0.5774 mL	2.8871 mL	5.7742 mL	14.4354 mL
	10 mM	0.2887 mL	1.4435 mL	2.8871 mL	7.2177 mL
	15 mM	0.1925 mL	0.9624 mL	1.9247 mL	4.8118 mL
	20 mM	0.1444 mL	0.7218 mL	1.4435 mL	3.6089 mL
	25 mM	0.1155 mL	0.5774 mL	1.1548 mL	2.8871 mL
	30 mM	0.0962 mL	0.4812 mL	0.9624 mL	2.4059 mL
	40 mM	0.0722 mL	0.3609 mL	0.7218 mL	1.8044 mL
	50 mM	0.0577 mL	0.2887 mL	0.5774 mL	1.4435 mL
	60 mM	0.0481 mL	0.2406 mL	0.4812 mL	1.2030 mL
	80 mM	0.0361 mL	0.1804 mL	0.3609 mL	0.9022 mL
	100 mM	0.0289 mL	0.1444 mL	0.2887 mL	0.7218 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

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Gibberellic acid [77-06-5]

Katalog-Nummer HY-N1964-1ml

Contact local distributor :

Marke : MedChemExpress

Contact local distributor :

Other Forms of Gibberellic acid:

Gibberellic acid Related Antibodies

Molaritätsrechner

Verdünnungsrechner

Complete Stock Solution Preparation Table

Keywords:

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