Specifications

Product Data
Applications	IF, IHC, WB
Recommended Dilution	Western blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200.  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivities	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of FoxO3 protein when phosphorylated at Serine 253
Formulation	Phosphate buffered saline (PBS), pH~7.2, 0.02% sodium azide, 50% glycerol State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Predicted Protein Size	~97 kDa
Gene Name	forkhead box O3
Database Link	Entrez Gene 56484 MouseEntrez Gene 2309 Human UniProt ID O43524
Background	FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Forkhead transcription factors of the FoxO subfamily are emerging as a shared component among pathways regulating diverse cellular functions, such as differentiation, metabolism, proliferation, and survival. Their transcriptional output is controlled via a two-tiered mechanism of phosphorylation and acetylation.FOXO3A is a transcriptional activator which triggers apoptosis in the absence of survival factors. It recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. FOXO3a has been shown to induce cell growth arrest to effect tumor suppression and FOXO3A genotype is strongly associated with human longevity.
Synonyms	FOXO3A, AF6q21 protein
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Chemokine signaling pathway, Endometrial cancer, Neurotrophin signaling pathway, Non-small cell lung cancer