FlyCut^® HindIII is expressed and purified from E.coli that carries the recombinant HindIII gene. The molecular weight is 35.8 kDa, with the recognition site at A^AGCTT. The reaction is conducted for 5-15 minutes at 37°C, and heat-inactivated at 65°C for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation. 

Enzyme Properties 

Fast digestion in 5-15 minutes with high fidelity 

Application 

Genomic DNA, plasmid DNA, PCR product