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> Fab Anti-RABBIT IgG (H&L) (GOAT) Antibody Biotin Conjugated

Fab Anti-RABBIT IgG (H&L) (GOAT) Antibody Biotin Conjugated

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Katalog-Nummer 811-1602

Size : 1mg

Marke : Rockland Immunochemicals

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Telefonnummer : +1 850 650 7790

Background

Fab Anti-Rabbit IgG (H&L) Antibody generated in goat detects immunoglobulin g from rabbit, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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Specifications for Fab Rabbit IgG (H&L) Antibody Biotin Conjugated

Product Details

Goat Fab Anti-Rabbit IgG (H&L) Antibody Biotin Conjugated - 811-1602
Goat Fab Anti-Rabbit IgG Biotin Conjugated Antibody, Goat Fab Fragment Anti-Rabbit IgG Antibody Biotin Conjugation
Goat
IgG (H&L)
Biotin
Polyclonal
IgG Fab

Target Details

Rabbit
Rabbit IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum.  No reaction was observed against anti-Papain or anti-Goat IgG F(c).

Application Details

ELISA
IF, IHC, Multiplex  - View References
Fab Anti-Rabbit IgG Biotin Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Formulation

Lyophilized
1.0mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.

References (13)

(). Differential localization of histone variant TH2B during the first round compared with subsequent rounds of spermatogenesis. Dev Dyn.
Applications
IF, Confocal Microscopy
PubMed
(). GABAergic signaling in the pulmonary neuroepithelial body microenvironment: functional imaging in GAD67-GFP mice. Histochem Cell Biol.
Applications
IHC, ICC, Histology
PubMed
(). Precision-cut vibratome slices allow functional live cell imaging of the pulmonary neuroepithelial body microenvironment in fetal mice. Adv Exp Med Biol.
Applications
Undefined
PubMed
(). Expression of mechanogated two-pore domain potassium channels in mouse lungs: special reference to mechanosensory airway receptors. Histochem Cell Biol.
Applications
IHC, ICC, Histology; Multiplex Assay
PubMed
(). Neurotrophin-4 dependency of intraepithelial vagal sensory nerve terminals that selectively contact pulmonary NEBs in mice. Histol Histopathol.
Applications
IHC, ICC, Histology
PubMed
(). Neurochemical pattern of the complex innervation of neuroepithelial bodies in mouse lungs. Histochem Cell Biol.
Applications
IHC, ICC, Histology; Multiplex Assay
PubMed
(). Pulmonary expression of voltage-gated calcium channels: special reference to sensory airway receptors. Histochem Cell Biol.
Applications
IHC, ICC, Histology; Multiplex Assay
PubMed
(). Neurochemical characterisation of sensory receptors in airway smooth muscle: comparison with pulmonary neuroepithelial bodies. Histochem Cell Biol.
Applications
IF, Confocal Microscopy; Multiplex Assay
PubMed
(). Sensory receptors in the airways: neurochemical coding of smooth muscle-associated airway receptors and pulmonary neuroepithelial body innervation. Auton Neurosci.
Applications
IF, Confocal Microscopy; Multiplex Assay
PubMed
(). Quantification of neuroepithelial bodies and their innervation in fawn-hooded and Wistar rat lungs. Am J Respir Cell Mol Biol.
Applications
IHC, ICC, Histology; Multiplex Assay
PubMed
(). Vesicular glutamate transporter 2 is expressed in different nerve fibre populations that selectively contact pulmonary neuroepithelial bodies. Histochem Cell Biol.
Applications
IHC, ICC, Histology; Multiplex Assay
PubMed
(). The WFS1 gene, responsible for low frequency sensorineural hearing loss and Wolfram syndrome, is expressed in a variety of inner ear cells. Histochem Cell Biol.
Applications
IHC, ICC, Histology
PubMed
(). Triple immunofluorescence staining with antibodies raised in the same species to study the complex innervation pattern of intrapulmonary chemoreceptors. J Histochem Cytochem.
Applications
IHC, ICC, Histology; Multiplex Assay
PubMed

Related Protocols

ELISA Protocol
Heat-induced Antigen Retrieval Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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