F(ab')2 Rabbit IgG (H&L) Antibody Phycoerythrin conjugated

Specifications

Product Data
Applications	FC, IF
Recommended Dilution	IF: 1:100 - 1:250 FC: 1:100 - 1:250
Reactivities	Rabbit
Host	Goat
Clonality	Polyclonal
Immunogen	Rabbit IgG whole molecule
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit  IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation.  Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Concentration	0.5 mg/mL - lot specific
Conjugation	Phycoerythrin
Storage	Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Note	F(ab')2 Anti-Rabbit IgG Phycoerythrin Conjugated Secondary Antibody has been tested by dot blot and is ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.