EDG3 (S1PR3) (NM_005226) Human Tagged ORF Clone
CAT#: RC209253
S1PR3 (Myc-DDK-tagged)-Human sphingosine-1-phosphate receptor 3 (S1PR3)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
Product Images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | EDG3 |
| Synonyms | EDG-3; EDG3; LPB3; S1P3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | >RC209253 representing NM_005226 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAACTGCCCTCCCGCCGCGTCTCCAGCCGGTGCGGGGGAACGAGACCCTGCGGGAGCATTACCAGT ACGTGGGGAAGTTGGCGGGCAGGCTGAAGGAGGCCTCCGAGGGCAGCACGCTCACCACCGTGCTCTTCTT GGTCATCTGCAGCTTCATCGTCTTGGAGAACCTGATGGTTTTGATTGCCATCTGGAAAAACAATAAATTT CACAACCGCATGTACTTTTTCATTGGCAACCTGGCTCTCTGCGACCTGCTGGCCGGCATCGCTTACAAGG TCAACATTCTGATGTCTGGCAAGAAGACGTTCAGCCTGTCTCCCACGGTCTGGTTCCTCAGGGAGGGCAG TATGTTCGTGGCCCTTGGGGCGTCCACCTGCAGCTTACTGGCCATCGCCATCGAGCGGCACTTGACAATG ATCAAAATGAGGCCTTACGACGCCAACAAGAGGCACCGCGTCTTCCTCCTGATCGGGATGTGCTGGCTCA TTGCCTTCACGCTGGGCGCCCTGCCCATTCTGGGCTGGAACTGCCTGCACAATCTCCCTGACTGCTCTAC CATCCTGCCCCTCTACTCCAAGAAGTACATTGCCTTCTGCATCAGCATCTTCACGGCCATCCTGGTGACC ATCGTGATCCTCTACGCACGCATCTACTTCCTGGTGAAGTCCAGCAGCCGTAAGGTGGCCAACCACAACA ACTCGGAGCGGTCCATGGCACTGCTGCGGACCGTGGTGATTGTGGTGAGCGTGTTCATCGCCTGCTGGTC CCCACTCTTCATCCTCTTCCTCATTGATGTGGCCTGCAGGGTGCAGGCGTGCCCCATCCTCTTCAAGGCT CAGTGGTTCATCGTGTTGGCTGTGCTCAACTCCGCCATGAACCCGGTCATCTACACGCTGGCCAGCAAGG AGATGCGGCGGGCCTTCTTCCGTCTGGTCTGCAACTGCCTGGTCAGGGGACGGGGGGCCCGCGCCTCACC CATCCAGCCTGCGCTCGACCCAAGCAGAAGTAAATCAAGCAGCAGCAACAATAGCAGCCACTCTCCGAAG GTCAAGGAAGACCTGCCCCACACAGCCCCCTCATCCTGCATCATGGACAAGAACGCAGCACTTCAGAATG GGATCTTCTGCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209253 representing NM_005226 Red=Cloning site Green=Tags(s) MATALPPRLQPVRGNETLREHYQYVGKLAGRLKEASEGSTLTTVLFLVICSFIVLENLMVLIAIWKNNKF HNRMYFFIGNLALCDLLAGIAYKVNILMSGKKTFSLSPTVWFLREGSMFVALGASTCSLLAIAIERHLTM IKMRPYDANKRHRVFLLIGMCWLIAFTLGALPILGWNCLHNLPDCSTILPLYSKKYIAFCISIFTAILVT IVILYARIYFLVKSSSRKVANHNNSERSMALLRTVVIVVSVFIACWSPLFILFLIDVACRVQACPILFKA QWFIVLAVLNSAMNPVIYTLASKEMRRAFFRLVCNCLVRGRGARASPIQPALDPSRSKSSSSNNSSHSPK VKEDLPHTAPSSCIMDKNAALQNGIFCN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | CHROMATOGRAMS Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_005226 |
| ORF Size | 1134 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005226.4 |
| RefSeq Size | 4221 bp |
| RefSeq ORF | 1137 bp |
| Locus ID | 1903 |
| UniProt ID | Q99500 |
| Cytogenetics | 9q22.1 |
| Domains | 7tm_1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 42.1 kDa |
| Gene Summary | This gene encodes a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| RC209253L1 | Lenti ORF clone of Human sphingosine-1-phosphate receptor 3 (S1PR3), Myc-DDK-tagged | 986,00 EUR | |
| RC209253L2 | Lenti ORF clone of Human sphingosine-1-phosphate receptor 3 (S1PR3), mGFP tagged | 986,00 EUR | |
| RC209253L3 | Lenti ORF clone of Human sphingosine-1-phosphate receptor 3 (S1PR3), Myc-DDK-tagged | 986,00 EUR | |
| RC209253L4 | Lenti ORF clone of Human sphingosine-1-phosphate receptor 3 (S1PR3), mGFP tagged | 986,00 EUR | |
| RG209253 | S1PR3 (tGFP-tagged) - Human sphingosine-1-phosphate receptor 3 (S1PR3) | 886,00 EUR | |
| SC116857 | S1PR3 (untagged)-Human sphingosine-1-phosphate receptor 3 (S1PR3) | 686,00 EUR |