DNA Damage (8-OHdG) ELISA BioAssay™ Kit
Katalog-Nummer 474005-96T
Size : 96Tests
Marke : US Biological
Contact local distributor :
Telefonnummer : +1 850 650 7790
Shipping Temp
Blue IceStorage Temp
4°C/-20°CThe DNA Damage (8-OHdG) ELISA BioAssay™ Kit is a competitive assy for the qualification of 8-OHdG in serum, plasma, urine, culture media, cell lysates, tissue samples and saliva. This BioAssay™ kit offers a quick incubation time, stable reagents, and a user-friendly protocol. Analyze results using an absorbance plate reader.
Detection Range:
0.94-60ng/ml
Sensitivity:
0.59ng/ml
Precision:
Intra-assay CV: <5%
Inter-assay CV: <5%
Inter-assay CV: <5%
Test Principle:
This ELISA kit is a competitive assay that can be used for the quantification of 8-OHdG in serum, plasma, urine, culture media, cell lysates, tissue samples and saliva. The ELISA utilizes an 8-hydroxy-2-deoxyguanosine-coated plate and an HRP-conjugated 8-OHdG specific detection antibody. This produces an assay with a useful range of 0.94-60ng/ml, and a sensitivity of 0.59 ng/mL. Other highlights of this kit include a quick incubation time (60 minutes), stable reagents, and a user-friendly protocol.
It is important to note that the 8-OHdG antibody used in this assay recognizes both free 8-OHdG and DNA-incorporated 8-OHdG. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of DNA fragments and free 8-OHdG, concentrations of 8-OHdG reported by ELISA methodology will not coincide with those reported by LC-MS where the single nucleoside is typically measured. This shouldbe kept in mind when analyzing and interpreting experimental results.
Kit Components:
474005A: Microtiter Plate, 1x96wells
*474005B: 8-OHdG Standard (3.06ug/ml), 1x100ul
474005C: 8-OHdG (HRP) Mab Antibody, 1x75ul
474005D: Sample and Standard Diluent, 1x50ml
474005E: 8-OhdG Antibody Diluent, 1x13ml
474005F: Wash Buffer Concentrate, 10X, 1x50ml
474005G: TMB Substrate, 1x13ml
474005H: Stop Solution (1M HCl), 1x13ml
*474005B: 8-OHdG Standard (3.06ug/ml), 1x100ul
474005C: 8-OHdG (HRP) Mab Antibody, 1x75ul
474005D: Sample and Standard Diluent, 1x50ml
474005E: 8-OhdG Antibody Diluent, 1x13ml
474005F: Wash Buffer Concentrate, 10X, 1x50ml
474005G: TMB Substrate, 1x13ml
474005H: Stop Solution (1M HCl), 1x13ml
Storage and Stability:
Store *474005B powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.