CPB1 (Carboxypeptidase B1, Tissue, CPB, PCPB, PASP, Pancreas-specific protein)

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma.|The deduced 416aa preproenzyme (EC 3.4.17.2) has a 15aa signal peptide and a 95aa activation peptide. It shares 76% and 84% sequence identity with the rat and bovine homologs, respectively. DNA blot analysis indicated that the PCPB mRNA is transcribed from a single locus. Northern blot analysis demonstrated pancreas-specific expression.||Applications: |Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.||Recommended Dilution:|ELISA: 1:100-1:5000|Western Blot: 1:50-400|Immunocytochemistry: 1:50-500|Immunohistochemistry (frozen): 1:50-500|Immunohistochemistry (paraffin): 1:10-100|Optimal dilutions to be determined by the researcher.||AA Sequence:|MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ATGHSYEKYN KWETIEAWTQ QVATENPALI SRSVIGTTFE GRAIYLLKVG KAGQNKPAIF MDCGFHAREW ISPAFCQWFV REAVRTYGRE IQVTELLDKL DFYVLPVLNI DGYIYTWTKS RFWRKTRSTH TGSSCIGTDP NRNFDAGWCE IGASRNPCDE TYCGPAAESE KETKALADFI RNKLSSIKAY LTIHSYSQMM IYPYSYAYKL GENNAELNAL AKATVKELAS LHGTKYTYGP GATTIYPAAG GSDDWAYDQG IRYSFTFELR DTGRYGFLLP ESQIRATCEE TFLAIKYVAS YVLEHLY||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.