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> CHAPS [75621-03-3]

CHAPS [75621-03-3]

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Katalog-Nummer HY-15435-5g

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CHAPS, a derivative of Cholic acid, is a zwitterionic detergent for solubilizing membrane proteins. CHAPS is used for stabilization of various protein-DNA complexes and can retain biochemical activity of proteins in solution.

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CHAPS Chemische Struktur

CAS. Nr. : 75621-03-3

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CHAPS Related Antibodies

  • •J Colloid Interface Sci. 2019 May 1;543:96-105.  [Abstract]

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CHAPS, a derivative of Cholic acid, is a zwitterionic detergent for solubilizing membrane proteins. CHAPS is used for stabilization of various protein-DNA complexes and can retain biochemical activity of proteins in solution.

In Vitro

CHAPS (0.5 %) is able to stabilize complexes between DNA and DNA-binding factors such as AP-1, SPI, GATA-1 and α-regulated factor ISGF3, and retains their biochemical activity.
CHAPS can prevent dissociation of mononucleosomes diluted to sub-nanomolar concentrations.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CHAPS Related Antibodies

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

  • [1]. Kroflic A, et al. Thermodynamic characterization of 3-[(3-cholamidopropyl)-dimethylammonium]-1-propanesulfonate (CHAPS) micellization using isothermal titration calorimetry: temperature, salt, and pH dependence. Langmuir. 2012 Jul 17;28(28):10363-71.  [Content Brief]

    [2]. Menshikova I, et al. Nucleosomes structure and dynamics: effect of CHAPS. Int J Biochem Mol Biol. 2011;2(2):129-137.  [Content Brief]

  • [1]. Kroflic A, et al. Thermodynamic characterization of 3-[(3-cholamidopropyl)-dimethylammonium]-1-propanesulfonate (CHAPS) micellization using isothermal titration calorimetry: temperature, salt, and pH dependence. Langmuir. 2012 Jul 17;28(28):10363-71.

    [2]. Menshikova I, et al. Nucleosomes structure and dynamics: effect of CHAPS. Int J Biochem Mol Biol. 2011;2(2):129-137.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.6263 mL 8.1317 mL 16.2633 mL 40.6583 mL
5 mM 0.3253 mL 1.6263 mL 3.2527 mL 8.1317 mL
10 mM 0.1626 mL 0.8132 mL 1.6263 mL 4.0658 mL
15 mM 0.1084 mL 0.5421 mL 1.0842 mL 2.7106 mL
20 mM 0.0813 mL 0.4066 mL 0.8132 mL 2.0329 mL
25 mM 0.0651 mL 0.3253 mL 0.6505 mL 1.6263 mL
30 mM 0.0542 mL 0.2711 mL 0.5421 mL 1.3553 mL
40 mM 0.0407 mL 0.2033 mL 0.4066 mL 1.0165 mL
50 mM 0.0325 mL 0.1626 mL 0.3253 mL 0.8132 mL
60 mM 0.0271 mL 0.1355 mL 0.2711 mL 0.6776 mL
80 mM 0.0203 mL 0.1016 mL 0.2033 mL 0.5082 mL
100 mM 0.0163 mL 0.0813 mL 0.1626 mL 0.4066 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CHAPS75621-03-3OtherszwitterionicdetergentsurfactantproteincomplexesstabilizedInhibitorinhibitorinhibit

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