Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:
Western blot (WB)
Recommended dilution:
1: 1000 - 1: 3500 (WB)
Expected | apparent MW:
67 kDa
Reactivity
Confirmed reactivity:
Arabidopsis thaliana
Predicted reactivity:
Arabidopsis thaliana
Not reactive in:
Nicotiana benthamiana, Solanum lycopersicum
Application Examples
Application example
Total protein from 16-day old Arabidopsis thaliana Col-0 (wild-type) and cerk1-2 null mutant (Cao et al., 2014). Total protein was extracted using Buffer H (Heese et al., 2007; LaMontagne et al., 2016) except that 40 nM Calyculin was used. 70 µg of total protein was denatured at 65°C for 5 min, separated on an 8% SDS-PAGE and transferred for 1h using a tank transfer system to nitrocellulose membrane. Blots were blocked with 1x PBS (Fisher Scientific BP665-1) + 0.1 %Tween 20 (PBS-T) + 5% milk for 1.5h at room temperature (RT) with agitation. Blots were incubated with primary antibody at a dilution of 1: 1000 overnight at 4°C with agitation in 1x PBS-T + 5% milk. The antibody solution was decanted, and the blot washed four times (1x 5min and 3x 6min) in 1x PBS-T at RT with agitation. The blot was then incubated with secondary antibody (Goat anti Rabbit IgG (H&L)–HRP from Agrisera (
AS10 687 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. bentamiana, S. oleracea, S. lycopersicum, T. aestivum, Z. mays, V. vinifera
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