Cd80 rat monoclonal antibody, clone 1G10, Low Endotoxin
Specifications
Product Data
Clone Name
1G10
Application
FC, FN, IP
Recommended Dilution
Flow Cytometry. (Ref.2,7) Immunoprecipitation. (Ref.7) Functional Assays:In vivo and In vitro blocking of ligand binding. (Ref.7)
Reactivity
Mouse
Antibody Host
Rat
Isotype
IgG2a
Clonality
Monoclonal
Specificity
This antibody recognises CD80/B7-1 (Mr. 45-47 kDa). 1G10 blocks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigenpresenting cells. (Ref.2,7).
CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.
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