Specifications

Product Data
Clone Name	25.32
Applications	FC
Recommended Dilution	Flow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 1 x 10e6 cells in 100µl.
Reactivities	Bovine, Goat, Sheep
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Specificity	This antibody recognises the CD44 cell surface antigen, a 95kD glycoprotein expressed by most leucocytes and a subpopulation of thymocytes. In immunohistochemical staining, this antibody labels several cell types including medullary thymocytes, kupffer cells, kidney tubular epithelial cells and gut smooth muscle cells.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Database Link	UniProt ID Q29423
Background	The expression of CD44 is upregulated upon activation.
Synonyms	LHR, MDU2, MDU3, MIC4, CDw44, Epican, ECMR-III, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, PGP-1
Reference Data