Cd4 rat monoclonal antibody, clone GK1.5, Low Endotoxin
Specifications
Product Data
Clone Name
GK1.5
Application
FC, FN, IF, IHC, IP
Recommended Dilution
Flow cytometry: 1 μg/million cells. Immunoprecipitation: 1-2 μg/100-500 μg of protein in 1 ml lysate. Immunohistochemistry (frozen sections). Immunocytochemistry: 1-4 μg/ml. Functional application: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4.
Reactivity
Mouse
Antibody Host
Rat
Isotype
IgG2b
Clonality
Monoclonal
Immunogen
Mouse CTL clone V4 cells
Specificity
This antibody reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
