CD4 Mouse Monoclonal Antibody [Clone ID: 44.38]

Specifications

Product Data
Clone Name	44.38
Applications	FC
Recommended Dilution	Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 106 cells in 100 µl.
Reactivities	Goat, Sheep
Host	Mouse
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	Fetal thymocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3-NS1/1-Ag-4-1 myeloma cell line.
Specificity	This antibody recognises the Ovine CD4 cell surface glycoprotein, which is expressed by a subset of mature T lymphocytes.
Formulation	PBS Label: FITC State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Database Link	UniProt ID P05542
Background	CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Synonyms	T-cell surface antigen T4/Leu-3
Reference Data