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Calreticulin Antibody

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Katalog-Nummer ASM10388-STR-200

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Marke : Abcepta

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  • ICC/IF - Calreticulin Antibody ASM10388
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calreticulin Polyclonal Antibody (ASM10388). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calreticulin Polyclonal Antibody (ASM10388) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Calreticulin Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
  • WB - Calreticulin Antibody ASM10388
    Western blot analysis of multiple cell lines lysates showing detection of Calreticulin protein using Rabbit Anti-Calreticulin Polyclonal Antibody (ASM10388). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Calreticulin Polyclonal Antibody (ASM10388) at 1:5000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
  • IHC - Calreticulin Antibody ASM10388
    Immunohistochemistry analysis using Rabbit Anti-Calreticulin Polyclonal Antibody (ASM10388). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calreticulin Polyclonal Antibody (ASM10388) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic granule. Endoplasmic reticulum lumen.
  • ICC/IF - Calreticulin Antibody ASM10388
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calreticulin Polyclonal Antibody (ASM10388). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calreticulin Polyclonal Antibody (ASM10388) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Calreticulin Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, FCM, ICC/IF
Primary Accession P27797
Other Accession NP_004334.1
Host Rabbit
Reactivity Human, Mouse, Rat, Hamster, Monkey, Pig, Chicken, Bovine, Dog, Sheep, Guinea Pig
Clonality Polyclonal
Description Rabbit Anti-Human Calreticulin Polyclonal
Target/Specificity Detects ~63kDa.
Other Names CALR Antibody, Calregulin Antibody, cC1qR Antibody, CRP55 Antibody, ERp60 Antibody, HSCBP Antibody, RO Antibody, SSA Antibody, grp60 Antibody
Immunogen Human calreticulin synthetic peptide with a cysteine residue added and the peptide conjugated to KLH
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-122 was sufficient for detection of Calreticulin in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Cellular Localization Endoplasmic Reticulum | Endoplasmic Reticulum Lumen | Cytoplasm | Cell Surface | Extracellular Matrix | Sarcoplasmic Reticulum | Sarcoplasmic Reticulum Lumen