Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie
 
> Calnexin-CT Antibody

Calnexin-CT Antibody

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Katalog-Nummer ASM10440-STR-200

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Marke : Abcepta

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  • ICC/IF - Calnexin-CT Antibody ASM10440
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440) at 1:80 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Melanosome. Magnification: 100x. Heat Shocked at 42°C for 1h.
  • WB - Calnexin-CT Antibody ASM10440
    Western blot analysis of Rat tissue mix showing detection of Calnexin-CT protein using Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
  • IHC - Calnexin-CT Antibody ASM10440
    Immunohistochemistry analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Hair Follicles, Basal cells in epidermis, and second layer of epidermis.
  • ICC/IF - Calnexin-CT Antibody ASM10440
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear staining, cytoplasmic staining.
  • ICC/IF - Calnexin-CT Antibody ASM10440
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440) at 1:80 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Calnexin-CT Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, FCM, ICC/IF
Primary Accession P24643
Other Accession NP_001003232.1
Host Rabbit
Reactivity Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Chicken, Quail, Bovine, Xenopus, Dog, Sheep, Guinea Pig, Drosophila
Clonality Polyclonal
Description Rabbit Anti-Dog Calnexin-CT Polyclonal
Target/Specificity Detects the C-terminal domain of Calnexin ~90kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues.
Other Names Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody
Immunogen Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:2000 dilution of SPC-182 was sufficient for detection of Calnexin in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
Cellular Localization Endoplasmic Reticulum | Endoplasmic Reticulum Membrane | Melanosome