C2orf76 (NM_001017927) Human Tagged ORF Clone

CAT#: RC206786

C2orf76 (Myc-DDK-tagged)-Human chromosome 2 open reading frame 76 (C2orf76)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


Plasmid Map

Product Images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol C2orf76
Synonyms AIM29
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206786 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCTGGAGAAGTGACCATCACAGTTCGCCTCATCCGTTCCTTTGAACATCGCAATTTCAAACCTG
TAGTGTATCACGGAGTGAATTTGGACCAAACTGTAAAGGAATTTATCGTATTTCTAAAGCAAGATGTCCC
TTTAAGGACCAACCTGCCACCACCATTCAGAAATTATAAATATGATGCACTAAAGATTATTCATCAAGCA
CATAAATCAAAGACAAATGAACTTGTGTTGAGTTTGGAAGATGACGAAAGACTCCTGCTGAAAGAAGACA
GCACTCTGAAAGCAGCTGGAATCGCCAGTGAAACTGAAATTGCATTCTTCTGTGAAGAAGATTATAGGAA
CTACAAAGCTAATCCCATTTCATCCTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206786 protein sequence
Red=Cloning site Green=Tags(s)

MAPGEVTITVRLIRSFEHRNFKPVVYHGVNLDQTVKEFIVFLKQDVPLRTNLPPPFRNYKYDALKIIHQA
HKSKTNELVLSLEDDERLLLKEDSTLKAAGIASETEIAFFCEEDYRNYKANPISSW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001017927
ORF Size 378 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001017927.1, NP_001017927.1
RefSeq Size 950 bp
RefSeq ORF 381 bp
Locus ID 130355
UniProt ID Q3KRA6
Cytogenetics 2q14.2
MW 14.6 kDa

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