BMP-7 BioAssay™ ELISA Kit (Chinese Hamster)

Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene. The protein encoded by this gene is a member of the TGF-Beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Cell culture supernates, serum, tissue, bone tissue, plasma (EDTA or Heparin).

Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Chinese Hamster BMP-7.

<10pg/ml

31.2pg/ml-2000pg/ml

Recognizes natural and recombinant Chinese Hamster BMP-7. There is no detectable cross-reactivity with other relevant proteins.

This Chinese Hamster BMP-7 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for Chinese Hamster BMP-7 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO; Immunogen sequence: S292-H430) and test samples are added to the wells, a biotinylated detection specific for Chinese Hamster BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Chinese Hamster BMP-7 amount of sample captured in plate.

96-well plate precoated with anti-Chinese Hamster BMP7 antibody, 1x plate
Lyophilized recombinant Chinese Hamster BMP7 standard, 2x10ng/tube
Biotinylated anti-Chinese Hamster BMP7 antibody, 1x130ul (dilution 1:100)
Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)
Sample diluent buffer, 1x30ml
Antibody diluent buffer, 1x12ml
ABC diluent buffer, 1x12ml
TMB color developing agent, 1x10ml
TMB stop solution, 1x10ml
PBS washing buffer Powder for 1000ml, 1x buffer

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards