Avidin/Biotin Blocking Kit, BioAssay™
Katalog-Nummer A4500-14-1Kit
Size : 1Kit
Marke : US Biological
Contact local distributor :
Telefonnummer : +1 850 650 7790
Applications
IHCShipping Temp
Blue IceStorage Temp
4°CAvidin/Biotin Blocking Kit BioAssay™ is designed to eliminate background caused by endogenous avidin or biotin-like substances. The kit contains 18ml each of avidin and biotin solutions.
Kit Components:
A4500-14A: Avidin Solution, 1x18ml. Supplied as a liquid, 0.05% sodium azide
A4500-14B: Biotin Solution, 1x18ml. Supplied as a liquid, 0.05% sodium azide
A4500-14B: Biotin Solution, 1x18ml. Supplied as a liquid, 0.05% sodium azide
Protocol:
These blocking steps should be performed before the primary antibody incubation step.
1. Apply 100ul (2 drops) of A4500-14A to the tissue.
2. Incubate for 10 minutes at RT.
3. Rinse extensively with PBS.
4. Apply 100ul (2 drops) of A4500-14B to the tissue.
5. Incubate for 10 minutes at RT.
6. Rinse extensively with PBS.
The endogenous avidin/biotin activity is now blocked and the tissue section is ready for immunohistochemical staining.
1. Apply 100ul (2 drops) of A4500-14A to the tissue.
2. Incubate for 10 minutes at RT.
3. Rinse extensively with PBS.
4. Apply 100ul (2 drops) of A4500-14B to the tissue.
5. Incubate for 10 minutes at RT.
6. Rinse extensively with PBS.
The endogenous avidin/biotin activity is now blocked and the tissue section is ready for immunohistochemical staining.
Storage and Stability:
Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.