Anti-Rabbit IgG F(c) (Texas Red™ Conjugated) Pre-Adsorbed Secondary Antibody

Katalog-Nummer ASR2358

Size : 2mg

Marke : Abcepta

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  • WB - Anti-Rabbit IgG F(c)  (Texas Red™ Conjugated) Pre-Adsorbed Secondary Antibody ASR2358
    Western Blot of Anti-Rabbit IgG F(c) (GOAT) Antibody . Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule . Lane 2: Rabbit IgG F(ab) Fragment . Lane 3: Rabbit IgG F(c) Fragment . Lane 4: Rabbit IgM Whole Molecule . Lane 5: Normal Rabbit Serum . All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Product Information
Description Anti-RABBIT IgG F(c) (GOAT) Antibody Texas Red™ Conjugated (Min X Human Serum Proteins)
Host Goat
Conjugate Texas Red®
FP Value 2.2 moles Texas Red® per mole of IgG
Target Species Rabbit
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC
Application Note FLISA 1:10,000-1:50,000
IF Microscopy 1:1,000-1:5,000
FlowCytometry 1:500-1:2,500
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG F(c)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rabbit IgG F(c) fragment
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum.  No reaction was observed against Rabbit IgG F(ab’)2 or Human Serum Proteins.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.