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> Anti-PLK1 (CHICKEN) Antibody

Anti-PLK1 (CHICKEN) Antibody

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Katalog-Nummer 200-901-MH7S

Size : 25ul

Marke : Rockland Immunochemicals

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Telefonnummer : +1 850 650 7790

Background

Polo-like kinases (PLKs) are a family of four serine/threonine protein kinases that belongs to the CDC5/Polo subfamily and are critical regulators of cell cycle progression, mitosis, cytokinesis, elevated levels are found in many different types of cancer, and the DNA damage response. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. PLK-1, -2 and -3 are ubiquitously expressed, whereas PLK-4 is restricted to a few tissues. Diseases associated with PLK1 include Breast Cancer, Transient Tic Disorder, and Monoclonal Paraproteinemia. Anti-PLK1 Antibody is useful for researchers interested in Cancer Researcher, Cell Cycle Research, and Kinase Activity Research.

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Specifications for PLK1 Antibody

Product Details

Anti-PLK1 (CHICKEN) Antibody - 200-901-MH7
Chicken Anti-Polo Like Kinase 1 Antibody, Chicken Anti-PLK1 Antibody, Chicken PLK-1 Antibody, Serine/Threonine-Protein Kinase 13, Polo-Like Kinase 1, STPK13, PLK-1, PLK, Serine/Threonine-Protein Kinase PLK1, Cell Cycle Regulated Protein Kinase, Polo-Like Kinase (Drosophila), Polo (Drosophila)-Like Kinase, EC 2.7.11.21
Chicken
Polyclonal
IgY

Target Details

PLK1  - View All PLK1 Products
Human
Conjugated Peptide
Anti-PLK1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to an internal portion of human PLK1 conjugated to Keyhole Limpet Hemocyanin (KLH).
This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Reactivity occurs against human PLK1. A BLAST analysis was used to suggest cross reactivity with PLK1 protein from rat, mouse, and bovine sources based on 100% homology with the immunizing sequence.
UniProtKB - P53350
NCBI - NP_005021.2
GeneID - 5347

Application Details

IF, WB
Anti-PLK1 antibody has been tested in Western Blot and Immunofluorescence. Expect a band ~68.3kDa in appropriate cell lysates or tissues. Positive controls used are HEK293T or K-562 whole cell lysate in WB, and HeLa cells in IF. Specific conditions for reactivity should be optimized by the end user.

Formulation

Liquid (sterile filtered)
1.05 mg/ml by UV absorbance at 280 nm
0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol
Fluorescent Western Blotting Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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