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> Anti-OVALBUMIN (Hen Egg White) (RABBIT) Antibody

Anti-OVALBUMIN (Hen Egg White) (RABBIT) Antibody

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Katalog-Nummer 200-401-033S

Size : 25ul

Marke : Rockland Immunochemicals

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Telefonnummer : +1 850 650 7790

Background

Anti-Ovalbumin Antibody recognizes ovalbumin that is the main protein found in egg white. Ovalbumin makes up 55-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins, it does not undergo a conformational change upon proteolytic cleavage and is not a serine protease inhibitor. The function of ovalbumin is still unknown, although it is presumed to be a storage protein for tissue-specific, steroid hormone-induced gene expression. Ovalbumin is a key protein for biochemical investigations and processes and can be used as a control, blocking reagent, or antibody in assays such as ELISA, SDS-PAGE, IHC, Western Blot, and size exclusion chromatography.

Expand Background

Specifications for OVALBUMIN Antibody

Product Details

Anti-Ovalbumin (Hen Egg White) (RABBIT) Antibody (BULK ORDER) - 200-4133
rabbit anti-Ovalbumin Antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2, Hen Egg White
Rabbit
Polyclonal
IgG

Target Details

SERPINB14  - View All SERPINB14 Products
Chicken
Native Protein
Ovalbumin [Hen Egg White]
Rabbit Anti-Ovalbumin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].  Cross reactivity against Ovalbumin from other sources is unknown.
UniProtKB - P01012
NCBI - P01012.2
GeneID - 396058

Application Details

ELISA, WB
Biochemical Assay  - View References
Anti-Ovalbumin Antibody has been tested in ELISA and Western Blot. Although not tested this product could be useful in assays such as IHC and IP. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation

Lyophilized
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
5.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.

References (2)

(). Label-free monitoring of human IgG/anti-IgG recognition using bloch surface waves on 1D photonic crystals. Biosensors (Basel).
Applications
Other
PubMed
(). Peptide mass fingerprinting by matrix‐assisted laser desorption ionization mass spectrometry of proteins detected by immunostaining on nitrocellulose. Proteomics.
Applications
WB, IB, PCA
PubMed

Related Protocols

Adherent Cell Lysis Protocol
Biotin Binding Assay Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Histone Immunoblotting Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Peptide Competition Assay (PCA) Protocol
Sandwich ELISA Protocol for Collagen
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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