LC3 Monoclonal Antibody.

anti-LC3 EM
anti-LC3 FCM



Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3.


This LC3 antibody is validated for multiple applications (WB, ICC, IH, IP, FCM, Immuno-EM) and has numerous citations. This is a monoclonal antibody targeting LC3 for FCM, ICC, IP, WB.

More information about LC3 and autophagy can be found in our Blog. In addition you may download our Autophagy Pathway Poster.

Target: LC3
Product Type: Antibody
Size: 200 µg/100 µL
Application: FCM, ICC, IH, Immuno-EM, IP, WB
Research Area / Disease: Autophagy
Conjugate: Unlabeled
Antibody Type: Monoclonal
Clone Number: 4E12
Concentration: 2 mg/mL
Formulation: 200 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Isotype: IgG1 ĸ
Immunogen: Recombinant human LC3 (MAP1LC3B: 1-120 a.a.)
Host Species: Mouse
Species Reactivity: Hamster, Human, Mouse, Rat
Source: This antibody was purified from hybridoma (clone 4E12) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with the recombinant human LC3 [MAP1LC3B (1-120 aa)].
Reactivity: This antibody reacts with LC3 (MAP1LC3A, B) on Immunocytochemistry, Western blotting and Immunoprecipitation.
Storage Temperature: -20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.


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