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> Anti-IgG4 (HRP) Monoclonal Antibody

Anti-IgG4 (HRP) Monoclonal Antibody

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Katalog-Nummer I1904-89D-200ug

Size : 200ug

Marke : US Biological

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I1904-89D IgG4 (HRP)

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P01861
Isotype
IgG1
Grade
Affinity Purified
Applications
E IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:1000-1:20,000
Immunohistochemistry: Paraffin sections
Immunohistochemistry (Frozen): 1:40-1:80.
Optimal dilutions to be determined by the researcher.

Immunohistochemistry Positive Control:
Tonsil

Hybrdoma:
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H2426 (HP6025)|Host: mouse|Source: human|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 0.01% thimerosal. Labeled with Horseradish Peroxidase (HRP).|Purity: Purified by Protein A affinity chromatography from ascites.|Immunogen: Purified IgG4. |Specificity: Recognizes an epitope in the Fc region of human IgG4. No crossreactivity is observed with human IgG1, IgG2, IgG3, IgM or IgA isotypes. Species Crossreactivity: chimpanzee.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Purified IgG4.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% thimerosal. Labeled with Horseradish Peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography from ascites.
Specificity
Recognizes an epitope in the Fc region of human IgG4. No crossreactivity is observed with human IgG1, IgG2, IgG3, IgM or IgA isotypes. Species Crossreactivity: chimpanzee.
References
1. Jefferis, R. et al. (1985) Immunology Letters. 10: 223-252. 2. Agaimy, A. et al. (2010) Am J Surg Pathol. 34: 271-8. 3. Strehl, J.D. et al. (2011) . J Clin Pathol. Jan 12. 4. Black, C.M. et al. (1991) Immunology.72: 94-8. 5. Fernandez-Becerra, C. (2010) Malar J. 9: 29. 6. Lee, H.W. et al. (2008) Korean J Parasitol. 46: 195-8.