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> Anti-IgG4 (FITC) Monoclonal Antibody

Anti-IgG4 (FITC) Monoclonal Antibody

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Katalog-Nummer I1904-89B1-FITC-100ul

Size : 100ul

Marke : US Biological

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I1904-89B1-FITC IgG4 (FITC)

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P01861
Isotype
IgG1
Grade
Purified
Applications
FLISA IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in FLISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen and paraffin sections.
Optimal dilutions to be determined by the researcher.

Hybridoma:
SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H2426 (HP6025)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified|Immunogen: Purified human IgG4.|Specificity: Recognizes the heavy chain of human IgG4 at an epitope in the Fc region. Does not crossreact with IgG1, IgG2, IgG3, IgM or IgA. Species Crossreactivity: chimpanzee||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Purified human IgG4.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified
Specificity
Recognizes the heavy chain of human IgG4 at an epitope in the Fc region. Does not crossreact with IgG1, IgG2, IgG3, IgM or IgA. Species Crossreactivity: chimpanzee
References
1. Jefferis, R. et al. (1985). Immunology Letters. 10:223-252. 2. Agaimy, A. et al. (2010) Am J Surg Pathol. 34:271-8. 3. Strehl, J.D. et al. (2011) Numerous IgG4-positive plasma cells are ubiquitous in diverse localised non-specific chronic inflammatory conditions and need to be distinguished from IgG4-related systemic disorders. J Clin Pathol. Jan 12. 4. Black, C.M. et al. (1991) Immunology. 72:94-8. 5. Fernandez-Becerra, C. (2010) Malar J. 9:29. 6. Lee, H.W. et al. (2008) Korean J Parasitol. 46:195-8.