General Info
| Host: | Mouse |
| Applications: | WB/IHC/IF |
| Reactivity: | Human/Mouse/Rat/Cow/Chicken/Dog/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Mouse monoclonal antibody anti-Transcription initiation factor IIB is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: | Monoclonal |
| Conjugation: | Unconjugated |
| Formulation: | Liquid in 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) , 0.2% Sodium Azide and 50% Glycerol. |
| Purification: | Affinity purification |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000-1:2000IHC 1:500-1:1000IF 1:100-1:500 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
| Gene Symbol: | GTF2B |
| Gene ID: | 2959 |
| Uniprot ID: | TF2B_HUMAN |
| Specificity: | TFIIB Monoclonal Antibody detects endogenous levels of TFIIB protein. |
| Immunogen: | Purified recombinant human TFIIB protein fragments expressed in E.coli. |
Description
| Post Translational Modifications | Acetylated. Autoacetylated.autoacetylation at Lys-238 stimulates transcription activation. |
| Function | General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner.these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-GCGCGACGCC-3', and downstream (BREd), 5'-GATTGATGGTTGTG-3', of the TATA box element. Modulates transcription start site selection. Exhibits also autoacetyltransferase activity that contributes to the activated transcription. |
| Protein Name | Transcription Initiation Factor IibGeneral Transcription Factor TfiibS300-Ii |
| Database Links | Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-674695Reactome: R-HSA-6807505Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-75953Reactome: R-HSA-76042 |
| Cellular Localisation | NucleusChromosomeNon-Acetylated Form Colocalizes With Dna In The G0/1S And G2 Phases Of The Cell CycleBut Not During MitosisAcetylated Form Colocalizes At Transcriptionally Silent Mitotic Chromatids During Mitosis At MetaphaseAnaphaseAnd Telophase Phases Of The Cell Cycle |
| Alternative Antibody Names | Anti-Transcription Initiation Factor Iib antibodyAnti-General Transcription Factor Tfiib antibodyAnti-S300-Ii antibodyAnti-GTF2B antibodyAnti-TF2B antibodyAnti-TFIIB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance