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> Anti-FGF21 antibody [S7MR]

Anti-FGF21 antibody [S7MR]

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Katalog-Nummer NB-22-48386-200

Size : 200μL

Marke : Neo Biotech

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Telefonnummer : +1 850 650 7790

  • Western blot analysis of extracts of various cell lines, using FGF21 rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of mouse liver, using FGF21 rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded rat lung using FGF21 rabbit monoclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human thyroid cancer using FGF21 rabbit monoclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spleen using FGF21 rabbit monoclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

General Info

Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-FGF21 (29-209) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: FGF21
Gene ID: 26291
Uniprot ID: FGF21_HUMAN
Immunogen Region: 29-209
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-209 of human FGF21 (NP_061986.1).
Immunogen Sequence: HPIPDSSPLLQFGGQVRQRY LYTDDAQQTEAHLEIREDGT VGGAADQSPESLLQLKALKP GVIQILGVKTSRFLCQRPDG ALYGSLHFDPEACSFRELLL EDGYNVYQSEAHGLPLHLPG NKSPHRDPAPRGPARFLPLP GLPPALPEPPGILAPQPPDV GSSDPLSMVGPSQGRSPSYA S

Description

Function Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity.
Protein Name Fibroblast Growth Factor 21
Fgf-21
Database Links Reactome: R-HSA-189200
Reactome: R-HSA-8963889
Cellular Localisation Secreted
Alternative Antibody Names Anti-Fibroblast Growth Factor 21 antibody
Anti-Fgf-21 antibody
Anti-FGF21 antibody
Anti-UNQ3115 antibody
Anti-PRO10196 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance