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> Anti-Chicken IgG (H&L) (Alkaline Phosphatase Conjugated) (Pre-Adsorbed) Secondary Antibody

Anti-Chicken IgG (H&L) (Alkaline Phosphatase Conjugated) (Pre-Adsorbed) Secondary Antibody

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Katalog-Nummer ASR3041

Size : 1mg

Marke : Abcepta

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  • WB - Anti-Chicken IgG (H&L) (Alkaline Phosphatase Conjugated) (Pre-Adsorbed) Secondary Antibody ASR3041
    Western Blot of Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) . Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule . Lane 2: Chicken IgG F(c) Fragment . Lane 3: Chicken IgG F(ab) Fragment . Lane 4: Chicken IgM Whole Molecule . Lane 5: Chicken Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 72 kDa for Chicken IgG and Serum, 25 kDa for F(c) and F(ab), 75 kDa for IgM. Chicken F(c) migrates slightly higher.
Product Information
Description Anti-CHICKEN IgG (H&L) (DONKEY) Antibody Alkaline Phosphatase Conjugated (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins)
Host Donkey
Conjugate Alkaline Phosphatase (Calf Intestine)
Target Species Chicken
Reactivity Chicken
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Application Note ELISA 1:2,000-1:10,000
Western Blot 1:500-1:2,500
Immunohistochemistry 1:200-1:1,000
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Immunogen Chicken IgG whole molecule
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.1% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Chicken IgG and Chicken Serum.   No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.
Storage Condition Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.