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> Anti-CD4 (PE) Monoclonal Antibody

Anti-CD4 (PE) Monoclonal Antibody

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Katalog-Nummer 227420-100T

Size : 100Tests

Marke : US Biological

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227420 CD4 (PE)

Clone Type
Polyclonal
Host
mouse
Source
equine
Swiss Prot
F6Y6X8
Isotype
IgG1
Grade
Affinity Purified
Applications
FC
Crossreactivity
Eq
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

CD4 is a 58kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes. As in humans, equine CD4 expression is mutually exclusive with CD8 expression on mature T-cells||A study undertaken using CVS4 to identify CD4 on several wild african equid species indicates that CD4 recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra).||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: CVS4|Host: mouse|Source: equine|Concentration: Not Determined|Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% sucrose, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Equine Thymocytes.|Specificity: Recognizes equine CD4.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Equine Thymocytes.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% sucrose, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes equine CD4.
References
1. Lunn, D.P.et al. (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets. Immunol. 74: 251-257. 2. Kydd, J.H. and Antczak, D.F., (1991) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991 Equine Immunol. 4-5. 3. Deeg,C.A. et al. (2004) The uveitogenic potential of retinal S-antigen in horses. Invest Ophthalmol Vis Sci. 45: 2286-92 4. Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses. Can J Vet Res. 71: 213-7. 5. Brault, S.A. et al. (2010) The immune response of foals to natural infection with equid herpesvirus-2 and its association with febrile illness. Vet Immunol Immunopathol. 137: 136-41. 6. Goodman, L.B. et al. (2007) A point mutation in a herpesvirus polymerase determines neuropathogenicity. PLoS Pathog. 3(11):e160. 7. Hamza, E. et al. (2011) CD4(+)CD25(+) T cells expressing FoxP3 in Icelandic horses affected with insect bite hypersensitivity. Vet Immunol Immunopathol. Jun 6. [Epub ahead of print] 8. Go, Y.Y. et al. (2010) Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes. J Virol. 84: 4898-911. 9. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet Immunol Immunopathol. 62: 101-143. 10. Ibrahim, S. (2007) Analysis of monoclonal antibody cross-reactivity with leukocytes from equids and cloning of CD28. Anchor Text.