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> Anti-ALPHA-TUBULIN (MOUSE) Monoclonal Antibody

Anti-ALPHA-TUBULIN (MOUSE) Monoclonal Antibody

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Katalog-Nummer 200-301-880S

Size : 25ul

Marke : Rockland Immunochemicals

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Telefonnummer : +1 850 650 7790

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.

Expand Background

Specifications for Alpha-Tubulin Antibody

Product Details

Anti-Alpha-Tubulin (MOUSE) Monoclonal Antibody - 200-301-880
mouse anti-Alpha-Tubulin Antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control
Mouse
Monoclonal
IgG1

Target Details

TUBA1B  - View All TUBA1B Products
Human
Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
Anti-Tubulin Loading Control Antibody was purified by Protein A chromatography. This Loading Control antibody is directed against alpha tubulin.  A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
UniProtKB - P68363
NCBI - 17986283

Application Details

ELISA, IF, IHC, WB, Multiplex
Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.

Formulation

Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store Anti-Tubulin Antibody at -20° C prior to opening. Aliquot Loading Control Antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubulin Loading Control Antibody if not completely clear after standing at room temperature. This Anti-Tubulin Loading Control Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

References (18)

(). Characterization of the nuclear import of the human CHD4-NuRD complex. J Cell Sci.
Applications
WB, IB, PCA
PubMed
(). MYC activation impairs cell-intrinsic IFNγ signaling and confers resistance to anti-PD1/PD-L1 therapy in lung cancer. Cell Rep Med.
Applications
WB, IB, PCA
PubMed
(). TGFβ signaling is required for sclerotome resegmentation during development of the spinal column in Gallus gallus. Developmental Biology
Applications
WB, IB, PCA
PubMed
(). Human Cytomegalovirus miR-US25-1 Targets the GTPase RhoA To Inhibit CD34+ Hematopoietic Progenitor Cell Proliferation To Maintain the Latent Viral Genome. mBio.
Applications
IF, Confocal Microscopy
PubMed
(). TGFβ signaling is required for sclerotome resegmentation during development of the spinal column in Gallus gallus. bioRxiv Preprint
Applications
WB, IB, PCA
Source
(). A Novel miRNA-Mediated Mechanism to Retain Latent HCMV Genomes in Hematopoietic Progenitor Cells. Cell Press Preprint
Applications
Undefined
Source
(). A two-layered targeting mechanism underlies nuclear RNA sorting by the human exosome. Cell Rep.
Applications
WB, IB, PCA
PubMed
(). The RNA exosome shapes the expression of key protein-coding genes. Nucleic Acids Res.
Applications
WB, IB, PCA
PubMed
(). Canonical and noncanonical TGF-β signaling regulate fibrous tissue differentiation in the axial skeleton. Sci Rep.
Applications
WB, IB, PCA
PubMed
(). Uncovering the role of N-acetyl-aspartyl-glutamate as a glutamate reservoir in cancer. Cell Rep.
Applications
WB, IB, PCA
PubMed
(). Cardiac glycoside bufalin blocks cancer cell growth by inhibition of Aurora A and Aurora B activation via PI3K-Akt pathway. Oncotarget
Applications
IF, Confocal Microscopy
PubMed
(). Polycystin-1, the product of the polycystic kidney disease gene PKD1, is post-translationally modified by palmitoylation. Mol Biol Rep.
Applications
WB, IB, PCA
PubMed
(). SOX9 protein is stabilized by TGF-β and regulates PAPSS2 mRNA expression in chondrocytes. Osteoarthritis Cartilage
Applications
WB, IB, PCA
PubMed
(). The Common R71H-G230A-R293Q Human TMEM173 Is a Null Allele. Journal of Immunology
Applications
WB, IB, PCA
PubMed
(). Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation. Journal of Biological Chemistry
Applications
WB, IB, PCA
PubMed
(). CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes. Nature Structural & Molecular Biology
Applications
WB, IB, PCA
PubMed
(). Linking phenotypes and modes of action through high-content screen fingerprints. Assay Drug Dev Technol.
Applications
IF, Confocal Microscopy
PubMed
(). MPYS/STING-mediated TNF-α, not type I IFN, is essential for the mucosal adjuvant activity of (3′–5′)-cyclic-di-guanosine-monophosphate in vivo. J Immunol.
Applications
WB, IB, PCA
PubMed

Related Protocols

Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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