AMP Activated Protein Kinase alpha1 (AMPK a1) (PE) (Drosophila)
Katalog-Nummer A1475-01P4-PE-100ul
Size : 100ul
Marke : US Biological
Contact local distributor :
Telefonnummer : +1 850 650 7790
Host
mouseConjugate
PEIsotype
IgG2aClone Number
10B156 (84.1)Grade
Affinity PurifiedApplications
E IF IHC IP WBShipping Temp
Blue IceStorage Temp
4°C Do Not Freeze5 AMP activated protein kinase catalytic alpha 1 chain, 5' AMP activated protein kinase catalytic subunit alpha 1, AAPK1, AMPK alpha 1 chain, AMPK, AMPK1, AMPKa1, HMG CoA reductase kinase, MGC33776, MGC57364, PRKAA 1, PRKAA1, Protein kinase AMP activated alpha 1 catalytic subunit, Acetyl CoA carboxylase kinase, Hormone sensitive lipase kinase, Kinase AMPK alpha1
AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit, which is stabilised by non-catalytic regulatory beta- and gamma- subunits. AMPK plays a vital role in maintaining cellular energy homeostasis and is activated by rising levels of AMP under conditions of hypoxia, ischemia, exercise, low glucose levels.
AMPK may play an important role in the control of type 2 diabetes and metabolic syndromes. Recent studies suggest that AMPK functions as a suppressor of cell proliferation of both malignant and normal cells.
AMPK may play an important role in the control of type 2 diabetes and metabolic syndromes. Recent studies suggest that AMPK functions as a suppressor of cell proliferation of both malignant and normal cells.
Applications:
Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot: 1:100-1:1,000. Detects a band of ~63kD in rat brain cell lysates.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Product Type
Mab
Isotype
IgG2a
Clone No
10B156 (84.1)
Host
mouse
Source
Drosophila
Concentration
As Reported
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography.
Immunogen
Synthetic peptide corresponding to 29aa within the N-terminal catalytic domain of Drosophila AMPK alpha. Species Sequence Homology
Specificity
Recognizes both the alpha 1 and the alpha 2 isoforms of Drosophila AMP-activated protein kinase (AMPK).
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.