ASM10085-STR-200 | Alpha B Crystallin Antibody Biotrend

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 1A7.D5 (ASM10085). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody (ASM10085) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Alpha B Crystallin Antibody (D) Composite.
Western Blot analysis of Bovine cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 1A7.D5 (ASM10085). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody (ASM10085) at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, E, ICC/IF
Primary Accession	P02511
Other Accession	NP_001876.1
Host	Mouse
Isotype	IgG1
Reactivity	Human, Rat, Pig, Bovine
Clonality	Monoclonal
Description	Mouse Anti-Human Alpha B Crystallin Monoclonal IgG1
Target/Specificity	Detects ~20kDa (Predicted mol. weight is ~21kDa). Does not cross-react with αA-crystallin, βL-crystallin, βH-crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Other Names	AACRYA Antibody, CRYA2 Antibody, CRYAB Antibody, CTPP2 Antibody, HSPB5 Antibody, NY Ren 27 antigen Antibody, alpha crystallin BAntibody
Clone Names	1A7.D5
Immunogen	Native Alpha B Crystallin
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.2, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	0.5 µg/ml of SMC-159 was sufficient for detection of 50 ng purified alpha B crystalline by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Cytoplasm \| Nucleus

Alpha B Crystallin Antibody