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AHA1 Antibody

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Katalog-Nummer ASM10442-A633-100

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Marke : Abcepta

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Telefonnummer : +1 850 650 7790

 
  • ICC/IF - AHA1 Antibody ASM10442
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AHA1 Polyclonal Antibody (ASM10442). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AHA1 Polyclonal Antibody (ASM10442) at 1:60 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Endoplasmic reticulum. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-AHA1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
  • WB - AHA1 Antibody ASM10442
    Western blot analysis of multiple Cell line lysates showing detection of AHA1 protein using Rabbit Anti-AHA1 Polyclonal Antibody (ASM10442). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-AHA1 Polyclonal Antibody (ASM10442) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, ICC/IF
Primary Accession Q8BK64
Other Accession NP_666148.1
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Mouse AHA1 Polyclonal
Target/Specificity Detects ~ 38kDa.
Other Names Activator of 90 kDa heat shock protein ATPase homolog 1 antibody, Activator of heat shock 90kDa protein ATPase homolog 1 antibody, AHA 1 antibody, AHA1 antibody, AHSA 1 antibody, AHSA1 antibody, AHSA1_HUMAN antibody, C14orf3 antibody, HSPC322 antibody, p38 antibody
Immunogen Mouse Aha1
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µl/ml of SPC-183 was sufficient for detection of Aha1 in 10 µg of mixed human cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Endoplasmic Reticulum