AEBSF (hydrochloride) [30827-99-7]
Katalog-Nummer HY-12821-100mg
Size : 100mg
Marke : MedChemExpress
AEBSF hydrochloride is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, plasmin, thrombin, and trypsin.
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AEBSF hydrochloride Chemische Struktur
CAS. Nr. : 30827-99-7
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- Western blotting analysis of the expression level of pATF6, CHOP, Cleaved Caspase12 and Bax in shRNA-Cs1429 and shRNA-Cs1429+AEBSF cells.
- The proliferation capacity of shRNA-Cs1429 transfected cells, by treatment with AEBSF (200 μmol/l), is the highest among all the groups.
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AEBSF hydrochloride is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, plasmin, thrombin, and trypsin. | |
In Vitro | AEBSF inhibits the constitutive production of Aβ by directly inhibiting β-secretase in five different human cell lines, both neural and nonneural. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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The HeLa cells suspended in RPMI-1640 media containing 10% FCS are plated into each well of a 96-well microplate (5×103 cells/200 μL/well). After incubation for 24 h at 37°C, cells are treated with different doses of AEBSF (0, 25, 50, 100 μg/mL) for 48 h. Then, 20 μL fresh 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) reagent (5 μg/μL) is added into each well, and cells are cultured at 37°C in 5% CO2 for another 4 h. The media are discarded carefully, and 150 μL DMSO is added. Absorbance is read on a microplate reader at dual wavelengths of 540 and 620 nm. MCE hat die Genauigkeit dieser Methoden nicht unabhängig bestätigt. Sie dienen nur als Referenz. |
Mice injected with 2.5×103 parasites are randomLy assigned to one of the treatment groups according to the treatment given: without drugs (control group), vehicle alone (vehicle control group), pyrimethamine alone at different doses, LY311727 alone at different doses, AEBSF alone at different doses or AEBSF 76.8 mg/kg plus pyrimethamine 10 mg/kg. Each treatment group consists of 10 animals. Treatment is initiated 24 h after parasite inoculation and is continued for seven consecutive days. Mouse survival is monitored daily and continued in live mice until 15 days post-infection. All experiments are performed three times and the data shown represent the cumulative results. MCE hat die Genauigkeit dieser Methoden nicht unabhängig bestätigt. Sie dienen nur als Referenz. |
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[1]. Citron M, et al. Inhibition of amyloid beta-protein production in neural cells by the serine protease inhibitor AEBSF. Neuron. 1996 Jul;17(1):171-9
[2]. Nakabo Y, et al. Lysis of leukemic cells by human macrophages: inhibition by 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), a serine protease inhibitor. J Leukoc Biol. 1996 Sep;60(3):328-36.
[3]. Buitrago-Rey R, et al. Evaluation of two inhibitors of invasion: LY311727 [3-(3-acetamide-1-benzyl-2-ethyl-indolyl-5-oxy)propane phosphonic acid] and AEBSF [4-(2-aminoethyl)-benzenesulphonyl fluoride] in acute murine toxoplasmosis. J Antimicrob Chemother.
[4]. Jiang YH, et al. Serine protease inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) inhibits the rat embryo implantation in vivo and interferes with cell adhesion in vitro. Contraception. 2011 Dec;84(6):642-8.
* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO / H2O | 1 mM | 4.1721 mL | 20.8603 mL | 41.7206 mL | 104.3014 mL |
5 mM | 0.8344 mL | 4.1721 mL | 8.3441 mL | 20.8603 mL | |
10 mM | 0.4172 mL | 2.0860 mL | 4.1721 mL | 10.4301 mL | |
15 mM | 0.2781 mL | 1.3907 mL | 2.7814 mL | 6.9534 mL | |
20 mM | 0.2086 mL | 1.0430 mL | 2.0860 mL | 5.2151 mL | |
25 mM | 0.1669 mL | 0.8344 mL | 1.6688 mL | 4.1721 mL | |
30 mM | 0.1391 mL | 0.6953 mL | 1.3907 mL | 3.4767 mL | |
40 mM | 0.1043 mL | 0.5215 mL | 1.0430 mL | 2.6075 mL | |
50 mM | 0.0834 mL | 0.4172 mL | 0.8344 mL | 2.0860 mL | |
60 mM | 0.0695 mL | 0.3477 mL | 0.6953 mL | 1.7384 mL | |
80 mM | 0.0522 mL | 0.2608 mL | 0.5215 mL | 1.3038 mL | |
100 mM | 0.0417 mL | 0.2086 mL | 0.4172 mL | 1.0430 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
- Metabolic Enzyme/Protease Anti-infection
- Thrombin Influenza Virus Ser/Thr Protease
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
AEBSF30827-99-7ThrombinInfluenza VirusSer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesInhibitorinhibitorinhibit
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