ACTN3 Human shRNA Plasmid Kit (Locus ID 89)

Specifications

Product Data
Locus ID	89
Synonyms	ACTN3D
Vector	pRFP-C-RS
E. coli Selection	Chloramphenicol (34 ug/ml)
Mammalian Cell Selection	Puromycin
Format	Retroviral plasmids
Kit Components	ACTN3 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 89). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq	NM_001104, NM_001258371, NR_047663, NM_001104.1, NM_001104.2, NM_001104.3, NM_001258371.1, NM_001258371.2, BC099647, BC096323, BC099648, BC099649
UniProt ID	Q08043
Summary	This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Feb 2014]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact tech@biotrend.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at tech@biotrend.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).