8-Isoprostane (8-Iso-PGF2a) BioAssay™ ELISA Kit (Rat)
Katalog-Nummer 369695-96T
Size : 96Tests
Marke : US Biological
Contact local distributor :
Telefonnummer : +1 850 650 7790
Applications
EShipping Temp
Blue IceStorage Temp
4°C/-20°CThe 8-Isoprostane (8-iso-PGF2a) BioAssay™ ELISA Kit (Rat) is a quantitative competitive assay for the detection of 8-Isoprostane in rat serum, plasma, tissue homogenates and other biological fluids.
Detection Range:
15.25-1,000pg/ml
Sensitivity:
<19.375pg/ml
Precision:
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Inter-Assay CV: <10%
Assay Principle:
The microtiter plate provided in this kit has been pre-coated with 8-Isoprostane. 8-Isoprostane in the standard or sample competes with a fixed amount of plate-coated 8-Isoprostane for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin-HRP (SABC) is added to each well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of 8-Isoprostane in the samples is determined by comparing the absorbance of the samples to the standard curve.
Kit Components:
369695A: Microtiter Strips, 8x12 wells.
369695B: Standard, 2 vials
369695C: Sample/Standard Dilution Buffer, 1x20ml
369695D: Antibody-Biotin Concentrate, 1x160ul
369695E: Antibody Dilution Buffer, 1x10ml
369695F: Streptavidin-HRP Conjugate (SABC), 1x120ul
369695G: SABC Dilution Buffer, 1x10ml
369695H: TMB Substrate, 1x10ml
369695J: Stop Solution, 1x10ml
369695K: Wash Buffer (25X), 1x30ml
369695B: Standard, 2 vials
369695C: Sample/Standard Dilution Buffer, 1x20ml
369695D: Antibody-Biotin Concentrate, 1x160ul
369695E: Antibody Dilution Buffer, 1x10ml
369695F: Streptavidin-HRP Conjugate (SABC), 1x120ul
369695G: SABC Dilution Buffer, 1x10ml
369695H: TMB Substrate, 1x10ml
369695J: Stop Solution, 1x10ml
369695K: Wash Buffer (25X), 1x30ml
Storage and Stability:
Store unopened 369695A at 4°C; store at -20°C once opened. Store unopened 369695B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store 369695F and 369695H protected from light at 4°C. Store the rest of the components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Summary:
1. Wash plate 2 times before adding standards, samples and control (zero) to wells.
2. Add 50ul standard or sample to each well.
3. Immediately add 50ul Antibody-Biotin working solution to each well and incubate for 45 minutes at 37°C
4. Aspirate and wash 3 times.
5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C
6. Aspirate and wash 5 times.
7. Add 90ul TMB substrate. Incubate 15-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
9. Calculate results.
2. Add 50ul standard or sample to each well.
3. Immediately add 50ul Antibody-Biotin working solution to each well and incubate for 45 minutes at 37°C
4. Aspirate and wash 3 times.
5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C
6. Aspirate and wash 5 times.
7. Add 90ul TMB substrate. Incubate 15-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
9. Calculate results.
Source
Rat
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.