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> 5-Ph-IAA [168649-23-8]

5-Ph-IAA [168649-23-8]

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Katalog-Nummer HY-134653-5mg

Size : 5mg

Marke : MedChemExpress

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5-Ph-IAA is a derivative of IAA. 5-Ph-IAA, a ligand, establishes the auxin-inducible degron 2 (AID2) system together with an OsTIR1 (F74G) mutant. AID2 induces rapid and efficient depletion of mAID-fused proteins to study protein function in living cells, causing tumor suppression.

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5-Ph-IAA Chemische Struktur

5-Ph-IAA Chemische Struktur

CAS. Nr. : 168649-23-8

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5-Ph-IAA Related Antibodies

WB
    5-Ph-IAA (5 μM; 1 h) rapidly induces Zpr1 depletion (Fig A), leading to cell growth arrest (Fig B), in ZPR1-AID cells.

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    5-Ph-IAA is a derivative of IAA. 5-Ph-IAA, a ligand, establishes the auxin-inducible degron 2 (AID2) system together with an OsTIR1 (F74G) mutant. AID2 induces rapid and efficient depletion of mAID-fused proteins to study protein function in living cells, causing tumor suppression.

    In Vitro

    5-Ph-IAA (1 μM; 6 hours) leads to DHC1-mAC degradation in HCT116 cells constitutively expressing OsTIR1(F74G), which means the AID2 system better than the original AID system in generating conditional mutant cell lines.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    5-Ph-IAA Related Antibodies

    Western Blot Analysis

    Cell Line: HCT116 cells
    Concentration: 1 µM
    Incubation Time: 6 hours
    Result: Depleted DHC1-mAC efficiently in HCT116 cells constitutively expressing OsTIR1(F74G).
    In Vivo

    5-Ph-IAA (0-10 mg/kg; intraperitoneally injection; every day; 7 days), used for the AID2 system, is sufficient to deplete mAID-BRD4 and TOP2A-mAC, leading to tumour suppression.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Balb/c-nu female mice (7 weeks old; 16–20 g ) bearing mAID-BRD4 and TOP2A-mAC xenograft tumours cells
    Dosage: 0 mg/kg, 1 mg/kg, 3 mg/kg and 10 mg/kg
    Administration: Intraperitoneally injection; every day; 7 days
    Result: Displayed significant tumour suppression of mAID-BRD4 xenografts and TOP2A-mAC xenografts.

    251.28

    Solid

    C16H13NO2

    168649-23-8

    O=C(O)CC1=CNC2=C1C=C(C3=CC=CC=C3)C=C2

    Room temperature in continental US; may vary elsewhere.
    In Vitro: 

    DMSO : 100 mg/mL (397.96 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.9796 mL 19.8981 mL 39.7962 mL
    5 mM 0.7959 mL 3.9796 mL 7.9592 mL
    10 mM 0.3980 mL 1.9898 mL 3.9796 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (8.28 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (8.28 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (8.28 mM); Clear solution

    • 4.

      Add each solvent one by one:  PBS

      Solubility: 1 mg/mL (3.98 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    *All of the co-solvents are available by MCE.

    • [1]. Aisha Yesbolatova, et al. The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat Commun. 2020 Nov 11;11(1):5701.  [Content Brief]

    • [1]. Aisha Yesbolatova, et al. The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat Commun. 2020 Nov 11;11(1):5701.

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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