10X PBS pH 7.2 with Azide
Katalog-Nummer OORA00260
Size : 1L
Marke : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for OORA00260 |
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Product Format | Liquid (sterile filtered) |
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Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot |
Reconstitution and Storage | Store container at room temperature (18 to 26°C). If desired, the solution may be stored at 4°C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18 to 26°C) to restore solubility of some salts. Expiration date is one (1) year from date of opening. |
Concentration | 10X |
Specificity | Tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria. |
Application Info | 10X PBS pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% (w/v) Sodium Azide) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X PBS pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% (w/v) Sodium Azide) is meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water. |
Purification | Aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. |
Description of Target | Phosphate Buffered Saline (PBS) is a regularly used buffer in biological research. The phosphate ions help maintain a constant pH around neutral. The osmolarity of solution typically matches that of the human body (isotonic solution). PBS can act as a diluent for many experimental preparations and is non-toxic to cells. This preparation contains 0.1% sodium azide as a preservative and is 10X concentrated to be diluted 10-fold to the working concentration. |
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