Cholesterol [57-88-5]

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Cholesterol is the major sterol in mammals. It is making up 20-25% of structural component of the plasma membrane. Plasma membranes are highly permeable to water but relatively impermeable to ions and protons. Cholesterol plays an important role in determining the fluidity and permeability characteristics of the membrane as well as the function of both the transporters and signaling proteins. Cholesterol is also an endogenous estrogen-related receptor α (ERRα) agonist.

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Cholesterol Chemische Struktur

CAS. Nr. : 57-88-5

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Cholesterol is the major sterol in mammals. It is making up 20-25% of structural component of the plasma membrane. Plasma membranes are highly permeable to water but relatively impermeable to ions and protons. Cholesterol plays an important role in determining the fluidity and permeability characteristics of the membrane as well as the function of both the transporters and signaling proteins. Cholesterol is also an endogenous estrogen-related receptor α (ERRα) agonist.

Microbial Metabolite

 

Human Endogenous Metabolite

 

In Vitro

GT1-7 hypothalamic cells subjected to Cholesterol depletion in vitro produced 20-31% reductions in cellular Cholesterol content. All Cholesterol-depleted neuron-derived cells, exhibit decreased phosphorylation/activation of IRS-1 and AKT following stimulation by insulin, insulin-like growth factor-1, or the neurotrophins (NGF and BDNF). Reduction in cellular Cholesterol also results in increased basal autophagy and impairment of induction of autophagy by glucose deprivation.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Cholesterol can be used in animal modeling to construct a rat model of hyperlipidemia.

Insulin-deficient diabetes in mice can lead to a reduction in brain Cholesterol synthesis, which occurs through a change in expression of Cholesterol synthesis enzymes and their upstream regulators SREBP2 and SCAP1.
The pool of Cholesterol in the whole animal is 2,200 mg/kg body weight. This is true for essentially all species from the mouse to the primate and indicates that the average concentration of Cholesterol in the whole animal is 2.2 mg/g fresh tissue.
The basal metabolic rate in the mouse is 170 kilocalories (kcal)/day/kg, and the flow of Cholesterol from all peripheral organs to the liver is greater than 100 mg/day/kg.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

386.65

C27H46O

57-88-5

Solid

White to off-white

O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3(16)[C@]2(16)CC[C@@]4(C)[C@@]3(16)CC[C@]4(16)[C@@H](CCCC(C)C)C

  • Steroids
  • Microorganisms
  • Endogenous metabolite

Room temperature in continental US; may vary elsewhere.

In Vitro: 

Ethanol : 20 mg/mL (51.73 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5863 mL 12.9316 mL 25.8632 mL
5 mM 0.5173 mL 2.5863 mL 5.1726 mL
10 mM 0.2586 mL 1.2932 mL 2.5863 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.43 mg/mL (3.70 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (14.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% EtOH    90% Corn Oil

    Solubility: ≥ 1.43 mg/mL (3.70 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (14.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
  • [1]. Casaburi I, et al. Cholesterol as an Endogenous ERRα Agonist: A New Perspective to Cancer Treatment. Front Endocrinol (Lausanne). 2018 Sep 11;9:525.  [Content Brief]

    [2]. Dietschy JM, et al. Thematic review series: brain Lipids. Cholesterol metabolism in the central nervous system during early development and in the mature animal. J Lipid Res. 2004 Aug;45(8):1375-97.  [Content Brief]

    [3]. Fukui K, et al. Effect of Cholesterol Reduction on Receptor Signaling in Neurons. J Biol Chem. 2015 Sep 14.  [Content Brief]

  • [1]. Casaburi I, et al. Cholesterol as an Endogenous ERRα Agonist: A New Perspective to Cancer Treatment. Front Endocrinol (Lausanne). 2018 Sep 11;9:525.

    [2]. Dietschy JM, et al. Thematic review series: brain Lipids. Cholesterol metabolism in the central nervous system during early development and in the mature animal. J Lipid Res. 2004 Aug;45(8):1375-97.

    [3]. Fukui K, et al. Effect of Cholesterol Reduction on Receptor Signaling in Neurons. J Biol Chem. 2015 Sep 14.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 2.5863 mL 12.9316 mL 25.8632 mL 64.6580 mL
5 mM 0.5173 mL 2.5863 mL 5.1726 mL 12.9316 mL
10 mM 0.2586 mL 1.2932 mL 2.5863 mL 6.4658 mL
15 mM 0.1724 mL 0.8621 mL 1.7242 mL 4.3105 mL
20 mM 0.1293 mL 0.6466 mL 1.2932 mL 3.2329 mL
25 mM 0.1035 mL 0.5173 mL 1.0345 mL 2.5863 mL
30 mM 0.0862 mL 0.4311 mL 0.8621 mL 2.1553 mL
40 mM 0.0647 mL 0.3233 mL 0.6466 mL 1.6164 mL
50 mM 0.0517 mL 0.2586 mL 0.5173 mL 1.2932 mL
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  • Induced Disease Models Products
  • Cardiovascular System Disease Models
  • Hyperlipidemia Models
  • Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease Anti-infection
  • Liposome Estrogen Receptor/ERR Endogenous Metabolite Bacterial
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cholesterol57-88-5LiposomeEstrogen Receptor/ERREndogenous MetaboliteBacterialDiabetesSREBP2insulinphosphorylationIRS-1AKTexocyticunesterifiedtransporterssignalingERRαInhibitorinhibitorinhibit

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