Mouse TNF-a ELISA Kit (96-well)

CAT#: EA100124

Mouse TNF-a ELISA Kit (96-well)

Size: 1 x 48 wells 1 x 96 wells


Product Images

Specifications

Product Data
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hour
Signal Colorimetric
Curve Range 0.02 - 1.0 ng/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Specificity Natural and recombinant Mouse TNF-a Ligand
Sensitivity 7pg/mL
Reactivities Mouse
Interference No significant interference observed with available related molecules.
Components
  • Aluminium pouches with a Microwell Plate coated with antibody to mouse TNF-a (8X12)
  • 2 vials mouse TNF-a Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse TNF-a antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
Background

The prototype ligand of theTNFsuperfamily, TNF-./TNFSF1A, is a pleiotropic cytokine that plays a central role in inflammation and apoptosis .TNF-. is produced by activated macrophages and other cell types including T and B cells, NK cells, LAK cells, astrocytes, endothelial cells, smooth muscle cells and some tumor cells .

Mouse TNF-. cDNA encodes a 235 amino acid (aa) residue type II membrane protein . The 156 aa residue soluble TNF-. is released from the C-terminus of the membrane-anchored TNF-. by proteolytic cleavage by a matrix metalloprotease .

The membrane-anchored form of TNF-. has been shown to have lytic activity and may also have an important role in intercellular communication . The biologically active TNF-. has been shown to exist as a trimer .TNF-. is reported to promote inflammatory cell infiltration by upregulating leukocyte adhesion molecules on endothelial cells, serve as a chemotactic agent for monocytes, and activate phagocyte killing mechanisms . Deficiencies in either TNF-. or its receptors can increase susceptibility to infection by intracellular pathogens . TNF- may also play a role in lymphoid tissue development. Knockout mice lack splenic B cell follicles and the ability to form germinal centers . Other potential physiological roles for TNF-. and its receptors include regulating the differentiation of hematopoietic stem and progenitor cells .

Gene Symbol Tnf
Gene ID 21926
Standard Curve
Representative standard curve for TNF-a ELISA. TNF-a was diluted in serial two-fold steps in Sample Diluent.

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