Mouse TGF-beta 3 ELISA Kit

Specifications

Product Data
Assay Type	Sandwich ELISA kit of Quantitative Detection for Mouse TGF-beta 3
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	31.2pg/ml-2000pg/ml
Sample Type	Cell culture supernates, serum, plasma(EDTA) and urine.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of activated mouse TGF-beta 3
Sensitivity	<10pg/ml
Reactivities	Mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti-mouse TGFb3 antibody | 1 Lyophilized recombinant mouse TGFb3standard | 10 ng/tube x 2 Biotinylated anti-mouse TGFb3 antibody | 100 ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100) Sample diluent buffer | 30ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Plate Sealers 4 x Wash Buffer (25x) 20 ml
Background	Transforming growth factor beta 3(TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix(ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin(CTNNB1) via nuclear phospho-Smad2 and Smad4.
Gene Symbol	Tgfb3
Gene ID	21809