Specifications

Product Data
Assay Type	Sandwich ELISA kit of Quantitative Detection for Mouse CD56/NCAM1
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	625pg/ml-40,000pg/ml
Sample Type	Cell culture supernates, serum and plasma(heparin, EDTA) .
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of mouse CD56
Sensitivity	<20pg/ml
Reactivities	Mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- mouse CD56 antibody 1 Lyophilized mouse CD56 standard 40ng/tubex2 Biotinylated anti- mouse CD56 antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
Gene Symbol	Ncam1
Gene ID	17967