Mouse Cadherin-2/N-Cadherin ELISA Kit

Specifications

Product Data
Assay Type	Sandwich ELISA kit of Quantitative Detection for Mouse Cadherin-2/N-Cadherin
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	0.78ng/ml-50ng/ml
Sample Type	Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of mouse Cadherin-2
Sensitivity	<20pg/ml
Reactivities	Mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- mouse Cadherin-2 antibody 1 Lyophilized recombinant mouse Cadherin-2 standard 50ng/tubex2 Biotinylated anti- mouse Cadherin-2 antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml Plate Sealers 4 x Wash Buffer 1 Powder Pack for 1000 ml
Background	Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
Gene Symbol	Cdh2
Gene ID	12558