CSPG4 ELISA Kit (Mouse)

Katalog-Nummer OKEH01518

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CSPG4 ELISA Kit (Mouse) (OKEH01518) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CSPG4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CSPG4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CSPG4 captured in well.
ELISA Kit Range9.38-600pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.4% (n = 20)
Mean Inter-assay CV%: < 10.5% (n = 20)
ELISA Kit Component
ComponentAmount
CSPG4 Microplate96 Wells (12 x 8 Well strips)
CSPG4 Lyophilized Standard2
100X Biotinylated CSPG4 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity4.71 pg/mL
SpecificityNatural and recombinant Mouse Chondroitin sulfate proteoglycan 4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCspg4
Gene Full Namechondroitin sulfate proteoglycan 4
Alias Symbols4732461B14Rik;A;AN2;AN2 proteoglycan;AN2/NG2 proteoglycan;chondroitin sulfate proteoglycan 4;chondroitin sulfate proteoglycan NG2;Csp;Cspg4a;nerve/glial antigen 2;NG;NG2;proteoglycan AN2.
NCBI Gene Id121021
Protein NameQ8VHY0
Description of TargetChondroitin sulfate proteoglycan 4
Uniprot IDhttps://www.uniprot.org/uniprot/Q8VHY0
Protein Accession #NP_620570.2
Nucleotide Accession #NM_139001.2
Protein Size (# AA)2327
Molecular Weight252 kDa

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Katalog-Nummer
Beschreibung
Cond.
Preis zzgl. MwSt.
OKEH02554
 96Wells