Kinase Substrates
eIF-4E; Protein Synthesis Initiation Factor 4E
GLO118 [1 mg, € 380,-]
Cap-binding protein involved in rate limiting step in protein synthesis. Undergoes phosphorylation at Ser209 in response to growth factors and other extracellular stimuli. Occurs as a complex with eIF-4BP’s and as part of the eIF-4F complex. Source human recombinant.
Purity > 90% by SDS-PAGE. Apparent Mr ~ 28 kDa. For eIF-4E phosphorylation site mutant preparations see initiation factors. For eIF-4E antibody, see antibodies.
PDC; Pyruvate Dehydrogenase Complex
GLO124 [10 mg, € 380,-]
Mitochondrial enzyme regulated by reversible phosphorylation. Catalyzes the oxidative decarboxylation of pyruvate. Purified preparations undergo phosphorylation because endogenous kinase is tightly associated with the complex. Source bovine heart.
Purity > 90% by SDS-PAGE. Specific activity ~ 12-18 mmol of NADH formed per min per mg of protein with pyruvate as substrate.
Apparent Mr ~ 8,500,000 by sedimentation equilibrium centrifugation. Subunit apparent Mr's ~ 74,000 (E2), 53,000 (E3), 41,000 (E1a), 48,000 (E3-BP) and 36,000 (E1b).
1. Methods Enzymol 166: 321
2. Methods Enzymol 89:376
BCKDH; Branched-Chain a-Keto Acid Dehydrogenase Complex
GLO125 [10 mg, € 380,-]
Mitochondial enzymes regulated by reversible phosphorylation. Catalyzes the oxidative decarboxylation of the branched-chain a-keto acids derived from the essential amino acids, leucine, isoleucine and valine. Purified preparations undergo phosphorylation because endogenous
kinase is tightly associated with the complex. Source bovine kidney. Purity > 90% by SDS-PAGE. Specific activity ~ 8-10 mmol of NADH formed per min per mg of protein with a-ketoisovalerate as substrate. Apparent Mr ~ 3,200,000 by sedimentation equilibrium centrifugation.
Subunit apparent Mr's 52,000 (E2), 46,000 (E1a), 35,000 (E1b). NEW! BCKDH kinase kit: see GLO125-01 protein kinases.
1. Methods Enzymol 166: 321
2. Methods Enzymol 166: 309
MBP; Myelin Basic Protein
GLO126 [1 mg, € 380,-]
Useful as substrate for many protein kinases and protein phosphatases. Source bovine brain. Purity > 90% by SDS-PAGE. Apparent Mr ~ 20-kDa.
J Biol Chem 258: 930
c-Jun; Transactivating Factor
GLO127 [1 mg, € 380,-]
Important proto-oncogene and transcription factor. Source recombinant human. Purity > 90% by SDS-PAGE. Apparent Mr ~ 39-kDa.
Al-Murrani S. W. K. et al. in press